1. Acheteur
1.1.
Acheteur
Nom officiel: Karolinska Institutet
2. Procédure
2.1.
Procédure
Titre: Multiphoton imaging system
Description: The research group of Stefanos Stagkourakis will carry out a project focused on all-optical investigations that provide the capability of imaging and manipulating neuronal activity at the same time. Specifically, multiphoton imaging of Ca2+ and voltage indicators will be used to study neuron activity on large neuron populations in vivo and in vitro. Successful pursuit of this work requires simultaneous recording of activity and manipulation through multiphoton photostimulation of pre-selected neuron ensembles to study a causal role of specific neuron subpopulations in behavior. A key component of the project is complementing the head-fixed in vivo neural activity and ensemble manipulation recordings with neuron activity imaging in freely moving animals through the Inscopix miniscope technology. In order to be able to carry out the research, KI needs to purchase an Multiphoton imaging system that meets the following requirements: a. The system must have a volumetric imaging solution which is decoupled from the photostimulation pathway while still offering a travel range that will allow imaging between planes of interest that are spatially separated by at least 390um in Z. This is needed to allow imaging and interrogation of the areas of interest across layers. Without that level of travel, simultaneous imaging and stimulation at multiple planes of interest would not possible where they are needed. Decoupling the photostimulation and imaging beams is necessary otherwise biological temporal resolution will not be observed due to the delay between stimulating, refocusing and imaging. b. The system must have a Spatial Light Modulator (SLM) to produce an axial resolution no larger than 10um. This is necessary to ensure that only the cells of interest are stimulated. Stimulation of multiple cells throughout a 3D volume is a key part of the project. If the axial resolution is larger than 10um, there is a risk that multiple cell layers can be stimulated instead of it being limited to the areas of interest c. It’s important that the objective of the system can be rotated, without sacrificing the SLM resolution, excitation, or collection efficiency. This is necessary when undertaking behavioral experiments as tilting the animal can cause it to behave unnaturally, a variable which is difficult to account for and one which can compromise the scientific integrity of the experiment itself. Maintaining this integrity should not come at the expense of signal otherwise power would need to be increased, causing unnecessary distress to the animal and a further experimental variable. d. The system must have detectors that are physically protected from any transmitted lightused for the imaging of brain slices. There must be a protection circuit in place that willnot allow any multiphoton detection to occur while the transmitted light source isactive. e. The research involves correlative studies between head-fixed and freely moving mouse models. Having access to a cloud-based software platform that can incorporate both data types is necessary as it enables faster, more efficient batch processing of computationally intense features. f. The system must be able to image and stimulate independently throughout the whole volume of a brain slice (up to 300µm depth) with a switching speed between planes of around 15ms. This speed and depth combination is especially valuable for capturing dynamic processes in 3D and for stimulating specific preselected brain populations with temporal precision. To KI’s knowledge there is no other supplier that offers a setup with proven equivalent functions. KI will sign a contract with Bruker after a standstill period of 10 days after publishing this Voluntary ex ante transparency notice.
Identifiant de la procédure: 829cdc2e-e836-42d5-93d7-0ec14c7cfdb0
Identifiant interne: 2-4528/2024
Type de procédure: Négociée sans mise en concurrence préalable
2.1.1.
Objet
Nature du marché: Fournitures
Nomenclature principale (cpv): 38510000 Microscopes
Nomenclature supplémentaire (cpv): 38515200 Microscope à fluorescence
2.1.2.
Lieu d’exécution
N’importe où
2.1.4.
Informations générales
Base juridique:
Directive 2014/24/UE
6 kap 14 § 2 p. LOU -
5. Lot
5.1.
Lot: LOT-0000
Titre: Multiphoton imaging system
Description: The research group of Stefanos Stagkourakis will carry out a project focused on all-optical investigations that provide the capability of imaging and manipulating neuronal activity at the same time. Specifically, multiphoton imaging of Ca2+ and voltage indicators will be used to study neuron activity on large neuron populations in vivo and in vitro. Successful pursuit of this work requires simultaneous recording of activity and manipulation through multiphoton photostimulation of pre-selected neuron ensembles to study a causal role of specific neuron subpopulations in behavior. A key component of the project is complementing the head-fixed in vivo neural activity and ensemble manipulation recordings with neuron activity imaging in freely moving animals through the Inscopix miniscope technology. In order to be able to carry out the research, KI needs to purchase an Multiphoton imaging system that meets the following requirements: a. The system must have a volumetric imaging solution which is decoupled from the photostimulation pathway while still offering a travel range that will allow imaging between planes of interest that are spatially separated by at least 390um in Z. This is needed to allow imaging and interrogation of the areas of interest across layers. Without that level of travel, simultaneous imaging and stimulation at multiple planes of interest would not possible where they are needed. Decoupling the photostimulation and imaging beams is necessary otherwise biological temporal resolution will not be observed due to the delay between stimulating, refocusing and imaging. b. The system must have a Spatial Light Modulator (SLM) to produce an axial resolution no larger than 10um. This is necessary to ensure that only the cells of interest are stimulated. Stimulation of multiple cells throughout a 3D volume is a key part of the project. If the axial resolution is larger than 10um, there is a risk that multiple cell layers can be stimulated instead of it being limited to the areas of interest c. It’s important that the objective of the system can be rotated, without sacrificing the SLM resolution, excitation, or collection efficiency. This is necessary when undertaking behavioral experiments as tilting the animal can cause it to behave unnaturally, a variable which is difficult to account for and one which can compromise the scientific integrity of the experiment itself. Maintaining this integrity should not come at the expense of signal otherwise power would need to be increased, causing unnecessary distress to the animal and a further experimental variable. d. The system must have detectors that are physically protected from any transmitted lightused for the imaging of brain slices. There must be a protection circuit in place that willnot allow any multiphoton detection to occur while the transmitted light source isactive. e. The research involves correlative studies between head-fixed and freely moving mouse models. Having access to a cloud-based software platform that can incorporate both data types is necessary as it enables faster, more efficient batch processing of computationally intense features. f. The system must be able to image and stimulate independently throughout the whole volume of a brain slice (up to 300µm depth) with a switching speed between planes of around 15ms. This speed and depth combination is especially valuable for capturing dynamic processes in 3D and for stimulating specific preselected brain populations with temporal precision. To KI’s knowledge there is no other supplier that offers a setup with proven equivalent functions. KI will sign a contract with Bruker after a standstill period of 10 days after publishing this Voluntary ex ante transparency notice.
Identifiant interne: 2-4528/2024
5.1.1.
Objet
Nature du marché: Fournitures
Nomenclature principale (cpv): 38510000 Microscopes
Nomenclature supplémentaire (cpv): 38515200 Microscope à fluorescence
5.1.2.
Lieu d’exécution
N’importe où
5.1.16.
Informations complémentaires, médiation et réexamen
Organisation chargée des procédures de recours: Förvaltningsrätten
TED eSender: Mercell Holding ASA
6. Résultats
Attribution directe:
Justification de l’attribution directe: Le marché ne peut être exécuté que par un opérateur économique particulier en raison d’une absence de concurrence pour des raisons techniques
6.1.
Résultat – Identifiants des lots: LOT-0000
6.1.2.
Informations sur les lauréats
Lauréat:
Nom officiel: Bruker France S.A.S
Offre:
Identifiant de l’offre: 2-4528/2024
Identifiant du lot ou groupe de lots: LOT-0000
Valeur de l'offre: 700 000,00 EUR
Informations relatives au marché:
Identifiant du marché: 2-4528/2024
Titre: Multiphoton imaging system
Date à laquelle le lauréat a été choisi: 05/12/2024
8. Organisations
8.1.
ORG-0001
Nom officiel: Karolinska Institutet
Numéro d’enregistrement: 2021002973
Département: Upphandlare
Adresse postale: Nobels väg 5
Ville: STOCKHOLM
Code postal: 17177
Pays: Suède
Point de contact: Hanna Glad
Téléphone: +46 08-52480000
Rôles de cette organisation:
Acheteur
8.1.
ORG-0002
Nom officiel: Förvaltningsrätten
Numéro d’enregistrement: 202100-2742
Ville: Stockholm
Code postal: 115 76
Subdivision pays (NUTS): Stockholms län (SE110)
Pays: Suède
Point de contact: Förvaltningsrätten i Stockholm
Téléphone: 08-561 680 00
Rôles de cette organisation:
Organisation chargée des procédures de recours
8.1.
ORG-0003
Nom officiel: Bruker France S.A.S
L’organisation est une personne physique
Numéro d’enregistrement: 311 020 911 00013
Département: Sale
Ville: Wissembourg
Pays: France
Point de contact: Pascale Nicolas/Fabienne Gandrillo
Téléphone: +33 1 72 86 61 00
Rôles de cette organisation:
Soumissionnaire
Lauréat de ces lots: LOT-0000
8.1.
ORG-0004
Nom officiel: Mercell Holding ASA
Numéro d’enregistrement: 980921565
Adresse postale: Askekroken 11
Ville: Oslo
Code postal: 0277
Subdivision pays (NUTS): Oslo (NO081)
Pays: Norvège
Point de contact: eSender
Téléphone: +47 21018800
Télécopieur: +47 21018801
Rôles de cette organisation:
TED eSender
11. Informations relatives à l’avis
11.1.
Informations relatives à l’avis
Identifiant/version de l’avis: bf72f5ad-7d3d-49bf-ba18-330c8f3f3350 - 01
Type de formulaire: Notification préalable d’attribution directe
Type d’avis: Avis en cas de transparence ex ante volontaire
Sous-type d’avis: 25
Date d’envoi de l’avis: 06/12/2024 15:01:36 (UTC)
Date d’envoi de l’avis (eSender): 06/12/2024 15:01:44 (UTC)
Langues dans lesquelles l’avis en question est officiellement disponible: anglais
11.2.
Informations relatives à la publication
Numéro de publication de l’avis: 754171-2024
Numéro de publication au JO S: 240/2024
Date de publication: 10/12/2024
